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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and therefore may possibly add to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two more splice variants were described but in depth nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified through the antibody.
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This information outlines more feasible optimization of cellular and immunoassay parameters to make certain the very best benefits are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to the hugely conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead into the temporal coordination of each and every mitotic event. Cyclin E types a posh with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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